A Dark Incubation Period Is Important for Agrobacterium-Mediated Transformation of Mature Internode Explants of Sweet Orange, Grapefruit, Citron, and a Citrange Rootstock
Identifieur interne : 001431 ( Main/Exploration ); précédent : 001430; suivant : 001432A Dark Incubation Period Is Important for Agrobacterium-Mediated Transformation of Mature Internode Explants of Sweet Orange, Grapefruit, Citron, and a Citrange Rootstock
Auteurs : Mizuri Marutani-Hert [États-Unis] ; Kim D. Bowman [États-Unis] ; Greg T. Mccollum [États-Unis] ; T. Erik Mirkov [États-Unis] ; Terence J. Evens [États-Unis] ; Randall P. Niedz [États-Unis]Source :
- PLoS ONE [ 1932-6203 ] ; 2012.
English descriptors
- KwdEn :
- Agrobacterium (physiology), Blotting, Southern, Citrus paradisi (genetics), Citrus paradisi (microbiology), Citrus sinensis (genetics), Citrus sinensis (microbiology), Culture Media, Darkness, Plant Roots (genetics), Plant Roots (microbiology), Plant Stems (genetics), Plant Stems (microbiology), Plants, Genetically Modified, Polymerase Chain Reaction, Regeneration, Transformation, Genetic, Trees (genetics), Trees (microbiology).
- MESH :
- chemical : Culture Media.
- genetics : Citrus paradisi, Citrus sinensis, Plant Roots, Plant Stems, Trees.
- microbiology : Citrus paradisi, Citrus sinensis, Plant Roots, Plant Stems, Trees.
- physiology : Agrobacterium.
- Blotting, Southern, Darkness, Plants, Genetically Modified, Polymerase Chain Reaction, Regeneration, Transformation, Genetic.
Abstract
Citrus has an extended juvenile phase and trees can take 2–20 years to transition to the adult reproductive phase and produce fruit. For citrus variety development this substantially prolongs the time before adult traits, such as fruit yield and quality, can be evaluated. Methods to transform tissue from mature citrus trees would shorten the evaluation period via the direct production of adult phase transgenic citrus trees.
Factors important for promoting shoot regeneration from internode explants from adult phase citrus trees were identified and included a dark incubation period and the use of the cytokinin zeatin riboside. Transgenic trees were produced from four citrus types including sweet orange, citron, grapefruit, and a trifoliate hybrid using the identified factors and factor settings.
The critical importance of a dark incubation period for shoot regeneration was established. These results confirm previous reports on the feasibility of transforming mature tissue from sweet orange and are the first to document the transformation of mature tissue from grapefruit, citron, and a trifoliate hybrid.
Url:
DOI: 10.1371/journal.pone.0047426
PubMed: 23082165
PubMed Central: 3474820
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A Dark Incubation Period Is Important for <italic>Agrobacterium</italic>-Mediated Transformation of Mature Internode Explants of Sweet Orange, Grapefruit, Citron, and a Citrange Rootstock</title><author><name sortKey="Marutani Hert, Mizuri" sort="Marutani Hert, Mizuri" uniqKey="Marutani Hert M" first="Mizuri" last="Marutani-Hert">Mizuri Marutani-Hert</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>U.S. Horticultural Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Ft. Pierce, Florida, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>U.S. Horticultural Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Ft. Pierce, Florida</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="Bowman, Kim D" sort="Bowman, Kim D" uniqKey="Bowman K" first="Kim D." last="Bowman">Kim D. Bowman</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>U.S. Horticultural Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Ft. Pierce, Florida, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>U.S. Horticultural Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Ft. Pierce, Florida</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="Mccollum, Greg T" sort="Mccollum, Greg T" uniqKey="Mccollum G" first="Greg T." last="Mccollum">Greg T. Mccollum</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>U.S. Horticultural Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Ft. Pierce, Florida, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>U.S. Horticultural Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Ft. Pierce, Florida</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="Mirkov, T Erik" sort="Mirkov, T Erik" uniqKey="Mirkov T" first="T. Erik" last="Mirkov">T. Erik Mirkov</name><affiliation wicri:level="2"><nlm:aff id="aff2"><addr-line>Department of Plant Pathology and Microbiology, Texas A&M University, Texas AgriLIFE Research, Weslaco, Texas, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant Pathology and Microbiology, Texas A&M University, Texas AgriLIFE Research, Weslaco, Texas</wicri:regionArea><placeName><region type="state">Texas</region></placeName></affiliation></author><author><name sortKey="Evens, Terence J" sort="Evens, Terence J" uniqKey="Evens T" first="Terence J." last="Evens">Terence J. Evens</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>U.S. Horticultural Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Ft. Pierce, Florida, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>U.S. Horticultural Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Ft. Pierce, Florida</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="Niedz, Randall P" sort="Niedz, Randall P" uniqKey="Niedz R" first="Randall P." last="Niedz">Randall P. Niedz</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>U.S. Horticultural Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Ft. Pierce, Florida, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>U.S. Horticultural Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Ft. 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Pierce, Florida, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>U.S. Horticultural Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Ft. Pierce, Florida</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="Bowman, Kim D" sort="Bowman, Kim D" uniqKey="Bowman K" first="Kim D." last="Bowman">Kim D. Bowman</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>U.S. Horticultural Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Ft. Pierce, Florida, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>U.S. Horticultural Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Ft. Pierce, Florida</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="Mccollum, Greg T" sort="Mccollum, Greg T" uniqKey="Mccollum G" first="Greg T." last="Mccollum">Greg T. Mccollum</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>U.S. Horticultural Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Ft. Pierce, Florida, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>U.S. Horticultural Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Ft. Pierce, Florida</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="Mirkov, T Erik" sort="Mirkov, T Erik" uniqKey="Mirkov T" first="T. Erik" last="Mirkov">T. Erik Mirkov</name><affiliation wicri:level="2"><nlm:aff id="aff2"><addr-line>Department of Plant Pathology and Microbiology, Texas A&M University, Texas AgriLIFE Research, Weslaco, Texas, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant Pathology and Microbiology, Texas A&M University, Texas AgriLIFE Research, Weslaco, Texas</wicri:regionArea><placeName><region type="state">Texas</region></placeName></affiliation></author><author><name sortKey="Evens, Terence J" sort="Evens, Terence J" uniqKey="Evens T" first="Terence J." last="Evens">Terence J. Evens</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>U.S. Horticultural Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Ft. Pierce, Florida, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>U.S. Horticultural Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Ft. Pierce, Florida</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="Niedz, Randall P" sort="Niedz, Randall P" uniqKey="Niedz R" first="Randall P." last="Niedz">Randall P. Niedz</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>U.S. Horticultural Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Ft. Pierce, Florida, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>U.S. Horticultural Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Ft. Pierce, Florida</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2012">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agrobacterium (physiology)</term><term>Blotting, Southern</term><term>Citrus paradisi (genetics)</term><term>Citrus paradisi (microbiology)</term><term>Citrus sinensis (genetics)</term><term>Citrus sinensis (microbiology)</term><term>Culture Media</term><term>Darkness</term><term>Plant Roots (genetics)</term><term>Plant Roots (microbiology)</term><term>Plant Stems (genetics)</term><term>Plant Stems (microbiology)</term><term>Plants, Genetically Modified</term><term>Polymerase Chain Reaction</term><term>Regeneration</term><term>Transformation, Genetic</term><term>Trees (genetics)</term><term>Trees (microbiology)</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Culture Media</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Citrus paradisi</term><term>Citrus sinensis</term><term>Plant Roots</term><term>Plant Stems</term><term>Trees</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Citrus paradisi</term><term>Citrus sinensis</term><term>Plant Roots</term><term>Plant Stems</term><term>Trees</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Agrobacterium</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Blotting, Southern</term><term>Darkness</term><term>Plants, Genetically Modified</term><term>Polymerase Chain Reaction</term><term>Regeneration</term><term>Transformation, Genetic</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>Citrus has an extended juvenile phase and trees can take 2–20 years to transition to the adult reproductive phase and produce fruit. For citrus variety development this substantially prolongs the time before adult traits, such as fruit yield and quality, can be evaluated. Methods to transform tissue from mature citrus trees would shorten the evaluation period via the direct production of adult phase transgenic citrus trees.</p></sec><sec><title>Methodology/Principal Findings</title><p>Factors important for promoting shoot regeneration from internode explants from adult phase citrus trees were identified and included a dark incubation period and the use of the cytokinin zeatin riboside. Transgenic trees were produced from four citrus types including sweet orange, citron, grapefruit, and a trifoliate hybrid using the identified factors and factor settings.</p></sec><sec><title>Significance</title><p>The critical importance of a dark incubation period for shoot regeneration was established. These results confirm previous reports on the feasibility of transforming mature tissue from sweet orange and are the first to document the transformation of mature tissue from grapefruit, citron, and a trifoliate hybrid.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Bove, Jm" uniqKey="Bove J">JM Bové</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hidaka, T" uniqKey="Hidaka T">T Hidaka</name></author><author><name sortKey="Omura, M" uniqKey="Omura M">M Omura</name></author><author><name sortKey="Ugaki, M" uniqKey="Ugaki M">M Ugaki</name></author><author><name sortKey="Tomiyama, M" uniqKey="Tomiyama M">M Tomiyama</name></author><author><name sortKey="Kato, A" uniqKey="Kato A">A Kato</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Moore, Ga" uniqKey="Moore G">GA Moore</name></author><author><name sortKey="Jacono, Cc" uniqKey="Jacono C">CC 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S" uniqKey="Fukai S">S Fukai</name></author><author><name sortKey="Kiyosue, T" uniqKey="Kiyosue T">T Kiyosue</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Saini, Hk" uniqKey="Saini H">HK Saini</name></author><author><name sortKey="Gill, Ms" uniqKey="Gill M">MS Gill</name></author><author><name sortKey="Gill, Mis" uniqKey="Gill M">MIS Gill</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Perez Tornero, O" uniqKey="Perez Tornero O">O Perez-Tornero</name></author><author><name sortKey="Tallon, Ci" uniqKey="Tallon C">CI Tallon</name></author><author><name sortKey="Porras, I" uniqKey="Porras I">I Porras</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Peixoto De Oliveira, Ml" uniqKey="Peixoto De Oliveira M">ML Peixoto de Oliveira</name></author><author><name sortKey="Costa, Mgc" uniqKey="Costa M">MGC Costa</name></author><author><name sortKey="Viana Da Silva, C" uniqKey="Viana Da Silva C">C Viana da Silva</name></author><author><name sortKey="Otoni, Wc" uniqKey="Otoni W">WC Otoni</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Floride</li><li>Texas</li></region></list><tree><country name="États-Unis"><region name="Floride"><name sortKey="Marutani Hert, Mizuri" sort="Marutani Hert, Mizuri" uniqKey="Marutani Hert M" first="Mizuri" last="Marutani-Hert">Mizuri Marutani-Hert</name></region><name sortKey="Bowman, Kim D" sort="Bowman, Kim D" uniqKey="Bowman K" first="Kim D." last="Bowman">Kim D. Bowman</name><name sortKey="Evens, Terence J" sort="Evens, Terence J" uniqKey="Evens T" first="Terence J." last="Evens">Terence J. Evens</name><name sortKey="Mccollum, Greg T" sort="Mccollum, Greg T" uniqKey="Mccollum G" first="Greg T." last="Mccollum">Greg T. Mccollum</name><name sortKey="Mirkov, T Erik" sort="Mirkov, T Erik" uniqKey="Mirkov T" first="T. Erik" last="Mirkov">T. Erik Mirkov</name><name sortKey="Niedz, Randall P" sort="Niedz, Randall P" uniqKey="Niedz R" first="Randall P." last="Niedz">Randall P. Niedz</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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